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| Nascimento, Fernanda Gatti de Oliveira; Barbosa, Fernando Cristino; Rodrigues, Renata Dias; Pereira, Heloisa Castro; Gomes, Lara Reis; de Souza, Rafael Rocha. |
| Foi comparada a eficiência de duas técnicas de coloração, Ziehl-Neelsen modificado e Safranina modificada, para Cryptosporidium sp. em amostras fecais de duas espécies de Jararacas criadas em cativeiro. Cryptosporidium é um protozoário de carácter cosmopolita, oportunista, que acomete varias espécies, classificado dentro do filo Apicomplexa, e suas espécies parasitam as microvilosidades das células epiteliais do trato gastrointestinal. A localização desse protozoário caracteriza-se por ser intracelular, porém extracitoplasmático. Diferentemente de outros organismos, nos quais as infecções por Cryptosporidium são autolimitantes em indivíduos imunocompetentes, a criptosporidiose em répteis é frequentemente crônica e pode ser letal para serpentes. A... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bothrops moojeni; Bothrops atrox; Oocistos. |
| Ano: 2014 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/23710 |
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| SILVA,Ageane Mota da; COLOMBINI,Mônica; MOURA-DA-SILVA,Ana Maria; SOUZA,Rodrigo Medeiros de; MONTEIRO,Wuelton Marcelo; BERNARDE,Paulo Sérgio. |
| ABSTRACT This study addresses the clinical and epidemiological aspects of envenoming cases resulting from snakebites treated at a hospital in Cruzeiro do Sul, in the upper Juruá River region, western Brazilian Amazonia. The specific identity of snakes that caused the envenomings was inferred (a) from the diagnosis of patient symptoms and signs upon hospital admission, (b) by enzyme immunoassay for detection of Bothrops atrox and Lachesis muta venom from serum samples taken from patients before antivenom therapy, or (c) by direct identification of the snake, when it was brought along to the hospital or photographed. There were 133 snakebites (76.2 cases per 100,000 inhabitants) registered during one year (July 2017 to June 2018). Most snakebites (88.7%)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Venomous animals; Snakes; Envenoming; Acre; Bothrops atrox; Enzyme immunoassay. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000100090 |
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| Three horse-derived antivenoms were tested for their ability to neutralize lethal, hemorrhagic, edema-forming, defibrinating and myotoxic activities induced by the venom of Bothrops atrox from Antioquia and Chocó (Colombia). The following antivenoms were used: a) polyvalent (crotaline) antivenom produced by Instituto Clodomiro Picado (Costa Rica), b) monovalent antibothropic antivenom produced by Instituto Nacional de Salud-INS (Bogotá), and c) a new monovalent anti-B. atrox antivenom produced with the venom of B. atrox from Antioquia and Chocó. The three antivenoms neutralized all toxic activities tested albeit with different potencies. The new monovalent anti-B. atrox antivenom showed the highest neutralizing ability against edema-forming and... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Bothrops atrox; Snake venom; Antivenom; Neutralization; Antioquia; Chocó. |
| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300011 |
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| Menaldo,Danilo L.; Jacob-Ferreira,Anna L.; Bernardes,Carolina P.; Cintra,Adélia C. O.; Sampaio,Suely V.. |
| Background Snake venoms are complex mixtures of inorganic and organic components, mainly proteins and peptides. Standardization of methods for isolating bioactive molecules from snake venoms is extremely difficult due to the complex and highly variable composition of venoms, which can be influenced by factors such as age and geographic location of the specimen. Therefore, this study aimed to standardize a simple purification methodology for obtaining a P-I class metalloprotease (MP) and an acidic phospholipase A2 (PLA 2 ) from Bothrops atroxvenom, and biochemically characterize these molecules to enable future functional studies.Methods To obtain the toxins of interest, a method has been standardized using consecutive isolation steps. The purity level of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Snake venoms; Bothrops atrox; Toxins; Metalloprotease; Phospholipase A2; Isolation; Characterization; Chromatography. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100337 |
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| Petretski,J.H.; Kanashiro,M.; Silva,C.P.; Alves,E.W.; Kipnis,T.L.. |
| This article describes the presence of two new forms of a thrombin-like enzyme, both with apparent molecular masses of 38 kDa, in Bothrops atrox venom. Both share the ability to cleave fibrinogen into fibrin and to digest casein. Both present identical Km on the substrate BApNA. Their N-terminal amino acid sequences are identical for 26 residues, sharing 80% homology with batroxobin and flavoxobin. Two groups of monoclonal antibodies (mAbs) raised against the purified enzyme forms recognized different epitopes of the putative corresponding enzymes present in B. atrox crude venom. On Western blotting analysis of B. atrox crude venom, mAbs 5DB2C8, 5AA10 and 5CF11, but not mAbs 6CC5 and 6AD2-G5, revealed two or more protein bands ranging from 25 to 38 kDa. By... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Venoms; Bothrops atrox; Enzymes; Thrombin-like enzymes; Blood coagulation system. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100005 |
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